Effect of saphenous vein space packing in conventional varicose vein surgery

Background: Varicose veins are common. The prevalence has been variously reported from as little as 2% to over 20% in population studies. Management of varicose vein disease has changed drastically over the past decades. Since its introduction, surgery has gone through several stages of evolution until the method of ligation with stripping eventually became and remained the standard for a long time. The objective was to study the effect on Saphenous vein space packing in conventional varicose vein surgery on intra-operative bleeding and post-operative patient recovery.Methods: A prospective comparative study was conducted in JSS Hospital, Mysuru from August 2016 to October 2018. A total of 60 patients were selected for the purpose of the study based on the inclusion and exclusion criteria. The patient was passed on post operative day 2, 4, 7 and 30. The study population was divided into 2 groups, Group 1: 30 patients undergoing varicose vein surgery with packing and Group 2: 30 patients undergoing traditional varicose vein surgery without packing.Results: On pre-operative evaluation of GSV diameter, the mean diameter was found to be 8.20±2.3 mm in the conventional group and 7.30±1.82 mm in vein packing technique. The pain was found to be more in conventional group than Saphenous vein packing technique and score was found to statistically significant between the two groups on day 2 and 4.Conclusions: Saphenous vein space packing is an easy, economical and effective way of reducing post-operative pain in conventional varicose vein surgery.

Binding of Cu(2+)-beta-cyclodextrin complex to calf thymus DNA.

Cu(2+)-beta-cyclodextrin (1:1) complex has been found by UV, fluorescence and CD spectroscopy, polarimetry and gel electrophoresis to bind reversibly to calf thymus DNA. Using UV the binding constant was found to be 45280 +/- 7100 M-1. The binding of the complex Cu(2+)-BCD with DNA was stronger than that of free Cu2+. However the ternary complex formed thus was destroyed by EDTA.

Interaction of hexadecyltrimethylammonium bromide with yeast cells.

The interaction of hexadecyltrimethylammonium bromide (CTAB) with two yeast cells, Kluyveromyces fragilis and Saccharomyces cerevisiae, has been studied. Strong binding of CTAB to the cell was inferred from 1H and 13C NMR studies, the probable site of binding being the cell-surface. 13C and 31P NMR studies have indicated facilitation of free passage of molecules from outside to inside the cell and vice versa on treatment with CTAB. 31P NMR studies showed that intracellular pH (pHi) was affected in presence of CTAB and the rate of exchange of H+ and PO4(-3) between outside and inside of the cell was 508 s-1. CTAB treatment of yeast cells also affected pH and conductance measurements of the cell-suspension. There was a marked difference in the pH changes around the critical micellar concentration (CMC) of CTAB. The observed pH changes were dependent on (i) CTAB concentration, (ii) pH of the cell-suspension and (iii) pK values of groups from molecules released from the cell. Also, it was shown that ionisation of phosphate diester from polar head groups of membranes constituting cell surface enhanced CTAB binding. Conductance measurements have shown that observed changes were independent of the concentration of yeast cells, but probably dependent on CMC of CTAB.

Determination of distances of sugar protons from Mn2+ in concanavalin A.

1H NMR spin-lattice relaxation time (T1) measurements have been carried out with various sugars, viz. methyl alpha-D-glucopyranoside (alpha-MeGluP), methyl beta-D-glucopyranoside (beta-MeGluP), methyl alpha-D-mannopyranoside (alpha-MeManP), maltose (4-O-alpha-D-glucopyranosyl-D-glucose), nigerose (3-O-alpha-D-glucopyranosyl-D-glucose), p-nitrophenyl alpha-maltoside (PNP-alpha-maltoside) and p-nitrophenyl beta-maltoside (PNP-beta-maltoside) to determine the distances of sugar protons from Mn2+ in concanavalin A (Con A). With a rotational correlation time of 1.58 x 10(-10) s determined, distances were calculated using Solomon-Bloembergen equation. The data obtained indicated differences in disposition of different groups in the binding site of Con A. An average value of about 10 A was obtained for the distances of sugar protons from Mn2+ in Con A. In the case of mono and disaccharides, the non-reducing end sugar unit was found to be closer to Mn2+ than the reducing end one.